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Subsequently, the RNA was reversely transcribed into cDNA using HiScript II Q RT SuperMix for qPCR (+ gDNA wiper) and amplified with ChamQ SYBR qPCR Master Mix (Vazyme BioTech, China) in a 10 L reaction volume

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Freshly isolated PHHs were cultured in a monolayer for 24 h, also as described above